Caenorhabditis elegans dpy-5 is a cuticle procollagen processed by a proprotein convertase |
| |
Authors: | C. Thacker J. A. Sheps A. M. Rose |
| |
Affiliation: | (1) Department of Medical Genetics, University of British Columbia, 419-2125 East Mall, Vancouver, B.C., V6T 1Z4, Canada;(2) BC Cancer Research Centre, British Columbia Cancer Agency, Vancouver, B.C., V5Z 1L3, Canada |
| |
Abstract: | Genetic analysis of the nematode Caenorhabditis elegans reveals that all dpy-5 alleles are dominant suppressors of bli-4 blistering. Molecular cloning of dpy-5 establishes that it encodes a cuticle procollagen, defects in which are responsible for the short-body, dumpy phenotype. The null mutation, e907 removes the entire coding region, whereas the dpy-5 reference allele, e61, contains a nonsense substitution. RT-PCR analysis and a dpy-5::gfp fusion show that dpy-5 is expressed only in hypodermal cells at all post-embryonic life-cycle stages. Variable expression of dpy-5 in V lineage-derived seam cells suggests an alternative regulatory mechanism in these cells. The dpy-5 gene product contains an Arg-X-X-Arg cleavage motif that could be recognized by a proprotein convertase, such as BLI-4. Mutation of this site cause a dominant dumpy phenotype suggesting Dpy-5 procollagen requires processing for normal cuticle production. Received 13 January 2006; accepted 23 March 2006 |
| |
Keywords: | Procollagen C. elegans suppressor blisters proprotein convertase |
本文献已被 PubMed SpringerLink 等数据库收录! |
|