含抗条锈病基因和黑麦碱基因(sec-1)的小麦-黑麦小片段易位系的鉴定

An elite wheat line 1R734 was selected from the selfed progeny lines(BC-2F-8)of the monosomic 1R addition lines between common wheat cultivar Mianyang 11 and an inbred rye line “baili”,with good resistance to stripe rust.APAGE(acid-polyacrylamide gel electrophoresis)analyses showed that the rye secalin bands were present in 1R734. However, genomic in situ hybridization(GISH)provided that the terminal regions of the short arm of a pair of wheat chromosomes showed yellowgreen fluorescent signals. It has been concluded that a small segment of rye chromosome 1R carrying the gene(s)for resistance to stripe rust and secalin(sec-1)has been transferred to wheat line 1R734. Besides, the use of monosomic addition lines for transferring the small segments of alien chromosomes into wheat is an effective approach.