(1) Institute of Experimental Ophthalmology and Laboratory of Microbiolog, University of Antwerp, UIA, B-2610 Antwerp, (Belgium)
Abstract:
Summary Sucrose, nycodenz, metrizamide and a mixture of equal volumes of sucrose and metrizamide were used as density gradient media for the isolation of retinal rod outer segments. The high osmolarity of sucrose had a strongly negative effect on the nature of the rod outer segments, whereas they were much better preserved using iodinated density gradient media such as nycodenz and metrizamide for their isolation.