二种中药复方对慢性心理应激大鼠行为及下丘脑-垂体-肾上腺轴的影响

目的：观察半夏厚朴汤、桂枝甘草龙骨牡蛎汤对慢性心理应激大鼠行为及下丘脑一垂体一肾上腺轴的影响。方法：将SD大鼠分为5组：正常组、模型Ⅰ组（慢性心理应激模型，不灌服生理盐水）、模型Ⅱ组（慢性心理应激模型，灌服生理盐水）、半夏厚朴汤组（灌服半夏厚朴汤水煎浓缩液）、桂枝甘草龙骨牡蛎汤组（灌服桂枝甘草龙骨牡蛎汤水煎浓缩液），连续灌服19d测试各组大鼠行为、下丘脑促肾上腺皮质激素释放激素（CRH）、血浆促肾上腺皮质激素（ACTH）及血清皮质酮（CORT）。结果：半夏厚朴汤组大鼠第11、19d爬格次数增加，第11d挣扎次数增加，CRH、ACTH降低；桂枝甘草龙骨牡蛎汤组大鼠第19d爬格次数增加，第19d静止时间缩短，CRH、ACTH降低。结论：半夏厚朴汤、桂枝甘草龙骨牡蛎汤可作用于下丘脑-垂体-肾上腺轴的不同环节，一定程度上影响慢性应激大鼠的行为方式，调节慢性应激大鼠的内分泌功能。

The effect of two kinds of traditional Chinese herbal compound on the behavior and hypothalamus-pituitary-adrenal gland axis of the chronic psychological stress rats

Aim: To study the effects of Banxia Houpu Soup and Guizhi Gancao Longgu Muli Soup on the behaviors and HPA axis of chronic psychological stress rats. Methods: SD rats were divided into 5 groups: normal group, model groups I and II, Banxia Houpu Soup group and Guizhi Gancao Longgu Muli Soup group. The behavior, the hypothalamus CRH, plasma ACTH and serum CORT(radio-immunity method) of all the rats were observed. Results: On the 11th day, the crossing times of model group I and the crossing times and struggle time of model group II changed obviously compared with those of normal group. On the 19th day, the crossing times and still time of model group I and II had remarkable differences, while the contents of CRH, ACTH of model group I and the contents of CRH, ACTH and CORT of model group II increased obviously compared with those of normal group. Compared with those of model group I and II, the crossing times on the 11th and19th days and the struggle times on the 11th day of Banxia Houpu Soup group increased obviously, while the contents of CRH and ACTH decreased; the crossing times on the 19th day increased, but the still time on the 19th day shortened and thecontents of ACTH and CRH decreased in Guizhi Longgu Muli Soup. Conclusion: Banxia Houpu Soup and Guizhi Longgu Muli Soup can affect the behavior and the endocrinal system of the chronic psychological stress rats in a certain level by through the action on the different sectors of the hypothalamus-pituitary-adrenal gland axis.