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| 以离子液体和三乙胺作流动相添加剂分离苦参中的生物碱 | |
| 引用本文： | 边敏,尹浩,张尊建.以离子液体和三乙胺作流动相添加剂分离苦参中的生物碱[J].南京工业大学学报(自然科学版),2012,34(1):117-120. |
| 作者姓名： | 边敏 尹浩 张尊建 |
| 作者单位： | 1. 中国药科大学分析测试中心,药物质量与安全预警教育部重点实验室,江苏南京210009;南京工业大学理学院,江苏南京210009 2. 南京工业大学理学院,江苏南京,210009 3. 中国药科大学分析测试中心,药物质量与安全预警教育部重点实验室,江苏南京210009 |
| 摘 要： | 分别用离子液体1-丁基-3-甲基咪唑四氟硼酸盐（BMIM BF4）和三乙胺（TEA）作流动相添加剂,用高效液相色谱法分离苦参中的生物碱,并分别考察添加剂浓度对组分保留和分离效率的影响,对两种添加剂的分离效果做了比较研究,同时对分离机制做了初步探讨。结果表明:离子液体作添加剂能够使苦参中的生物碱达到完全分离,减少试样的拖尾峰,改善峰形,提高分离效率。与TEA体系相比,生物碱的保留值降低幅度较小。在相同浓度下,分离效能较高。添加量提高时,组分的保留值减小。 |
| 关 键 词： | 离子液体 苦参碱 三乙胺 HPLC 添加剂 |
Separation of matrine-type alkaloids with ionic liquids and triethylamine as mobile phase additives |
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| BIAN Min , YIN Hao , ZHANG Zunjian.Separation of matrine-type alkaloids with ionic liquids and triethylamine as mobile phase additives[J].Journal of Nanjing University of Technology,2012,34(1):117-120. | |
| Authors: | BIAN Min YIN Hao ZHANG Zunjian |
| Institution: | 1 (1.Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education,Center for Instrumental Analysis,China Pharmaceutical University,Nanjing 210009,China; 2.College of Sciences,Nanjing University of Technology,Nanjing 210009,China) |
| Abstract: | The chromatographic behavior of matrine-type alkaloids on a C18 column containing different concentrations of 1-butyl-3-methylimidazolium tetrafluoroborate（BMIM BF4 ） or triethylamine（TEA） was studied by using retention factor and peak efficiency as the criteria for chromatographic efficiency. The result showed that the addition of BMIM BF4 had superior effects on the separation of these basic compounds with reducing tailing and improving peak shape.Meanwhile,BMIM BF4 didn’t change the solute retention factors and improve chromatographic efficiency while TEA decreased on same concentration. |
| Keywords: | ionic liquid matrine triethylamine HPLC additive |
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