| 茶多酚的加速溶剂萃取与分析 |
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| 引用本文： | 赵敏,唐美华,张之翼,吕凤兰,陈国松.茶多酚的加速溶剂萃取与分析[J].南京工业大学学报(自然科学版),2012,34(4):123-127. |
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| 作者姓名： | 赵敏 唐美华 张之翼 吕凤兰 陈国松 |
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| 作者单位： | 南京工业大学理学院,江苏南京,210009 |
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| 摘 要： | 以毛尖茶叶为原料,用加速溶剂萃取法提取茶多酚粗品用单因素试验研究和正交试验考察乙醇体积分数、温度和循环次数对萃取率的影响。用二极管阵列高效液相色谱(DAD-HPLC)对照测定加速溶剂萃取的茶多酚粗品、微波辅助提取的茶多酚粗品和商品茶多酚。结果表明:茶多酚粗品的制备最佳条件为萃取温度160℃、乙醇体积分数60%、循环萃取2次。高温加速溶剂萃取法对茶多酚的粗提率较高,但其中儿茶素的总量却显著下降降低提取温度,则茶多酚中的主要功能成分儿茶素的含量显著高于微波辅助提取法。
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| 关 键 词： | 茶多酚 儿茶素 加速溶剂萃取 二极管阵列-高效液相色谱法 |
Accelerated solvent extraction and analysis of tea polyphenols |
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| ZHAO Min
,
TANG Meihua
,
ZHANG Zhiyi
,
L Fenglan
,
CHEN Guosong.Accelerated solvent extraction and analysis of tea polyphenols[J].Journal of Nanjing University of Technology,2012,34(4):123-127. |
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| Authors: | ZHAO Min TANG Meihua ZHANG Zhiyi L Fenglan CHEN Guosong |
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| Institution: | ZHAO Min
,
TANG Meihua
,
ZHANG Zhiyi
,
L(U) Fenglan
,
CHEN Guosong |
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| Abstract: | The gross tea polyphenols were extracted from Maojian tea by the accelerated solvent method. Single factor test and orthogonal design were used to explore the effects of ethanol volume fraction,temperature and cycle times on the extraction ratio.The comparison of gross polyphenols got from accelerated solvent extraction,microwave-assisted extraction and commodity was made by diode array detector-high performance liquid chromatography(DAD-HPLC)analysis.Results showed that the optimum operation conditions for gross polyphenlos extraction are 160℃,2 times,and 60%ethanol.The accelerated solvent extraction ratio at a high temperature was higher for the gross tea polyphenols and lower for the catechin. If the extraction temperature was dropped down to 40℃,the content of catechin was much higher than that from microwave-assisted extraction. |
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| Keywords: | tea polyphenol catechin accelerated solvent extraction DPD-HPLC |
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