| 红芪组织培养中次生代谢产物的研究Ⅰ.红芪愈伤组织异柠檬酸裂解酶活性的变化 |
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| 引用本文： | 邢更妹,薛荣,王仑山.红芪组织培养中次生代谢产物的研究Ⅰ.红芪愈伤组织异柠檬酸裂解酶活性的变化[J].兰州大学学报(自然科学版),1999(2). |
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| 作者姓名： | 邢更妹 薛荣 王仑山 |
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| 作者单位： | 甘肃农业大学农学系(邢更妹)，解放军兰州医学高等专科学校(薛荣)，兰州大学生物系(王仑山) |
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| 摘 要： | 在红芪不同生长状态的愈伤组织中，异柠檬酸裂解酶（ＩＣＬ）活性差异很大，其中以分化愈伤组织中活性最高．与此相平行在红芪不同培养时期的愈伤组织中，不饱和脂肪酸含量降低，多糖含量升高．由此可以肯定异柠檬酸裂解酶为红芪次生代谢产生多糖过程中的一个重要酶．同时在无菌苗中异柠檬酸裂解酶活性也很高，而多糖没有检测到，说明异柠檬酸裂解酶也参加了植物细胞的初级代谢．我们认为初级和次级代谢过程中某些重要的甚至关键的酶是相同的，只是随着细胞的发育过程其活性发生了变化．
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| 关 键 词： | 红芪 愈伤组织 异柠檬酸裂解酶 不饱和脂肪酸 多糖 |
Change of the Isocitrate Lyase Activity in the Callus of H.Polybotrys Hand Mass |
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| Xing
Gengmei ,Xue Rong ,Wang Lunshan.Change of the Isocitrate Lyase Activity in the Callus of H.Polybotrys Hand Mass[J].Journal of Lanzhou University(Natural Science),1999(2). |
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| Authors: | Xing
Gengmei Xue Rong Wang Lunshan |
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| Institution: | Xing
Gengmei 1,Xue Rong 2,Wang Lunshan 3 |
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| Abstract: | In
the callus of H. Polybotrys Hand Mass in different states of growth, the isocitratev lyase
activity has a big divergence. It is the highest in the differentiating callus. The activity of
isocitrate lyase rises when the insaturated fatty acid drops. For this reason, a calculation is
given. Isocitrate lyase is an important enzyme in H.Polybotrys Hand Mass produced in its
secondary metabolism. In the bacteria free seed, the isocitrate lyase activity is high. It shows
that ICL has joined in primary metabolism. It seems to us that some of the important enzymes
produced in the primary and secondary metabolic activities are the same, but their activities
change with the growth of cells. |
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| Keywords: | H Polybotrys Hand Mass callus ICL insaturated fatty acid polysaccharose |
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