1 College of Science, National University of Defense Technology, Changsha 410073, China;
2 College of Aerospace and Material Engineering, National University of Defense Technology, Changsha 410073, China
Abstract:
Using tetraethoxysilane and 3-aminopropyltriethoxysilane as the silica sources, amino-functionalized mesoporous silica thin films with 3-dimensional cubic structure have been deposited on conducting ITO substrate in the presence of surfactant F127 templates under acid conditions. The acetylcholinesterase (AchE) and cytochrome c (Cyt c) were incorporated into the pores of mesoporous thin films, and an amperometric biosensor was obtained. After adsorption of AchE and Cyt c, the ordered cubic structure of meso...