细粒棘球绦虫AgB1抗原表位的生物信息学预测

 以细粒棘球绦虫AgB1基因序列为基础,采用PredictProtein软件预测其编码蛋白的二级结构;应用在线预测软件Bcepred、Abcpred、IEDB及SYFPEITHI等对细粒棘球绦虫AgB1的B细胞表位和T细胞表位进行预测。结果提示,AgB1抗原蛋白存在可以构成抗原表位的区域,经软件分析,分值高的B细胞表位区域:2~9、15~20、22~35和41~52氨基酸序列;T细胞表位区域:3~12、26~33、34~44和52~61氨基酸序列。研究运用生物信息学确定AgB1抗原的4个B细胞优势表位和4个T细胞优势表位,对进一步研究AgB1的抗原性和研发更有价值的免疫诊断方法具有重要意义。

Bioinformatics Prediction of AgB1 Antigen Epitopes of Echinococcus granulosus

The secondary structure, B cell and T cell epitopes for the AgB1 antigen protein of Echinococcus granulosus were predicted and analyzed. According to the gene sequences of AgB1 antigen, the protein secondary structure was analyzed by PredictProtein, and B cell and T cell epitopes of AgB1 were predicted by Internet website Bcepred, Abcpred, IEDB and SYFPEITHI. The results show that many distinct antigenic epitopes of AgB1 were identified by computation, and the high scored B cell epitopes in the regions of 2-9, 15-20, 22-35 and 41-52. The possible T cell epitopes is in the regions of 3-12, 26-33, 34-44 and 52-61. In this study it is determined that there are 4 B cell dominant epitopes and 4 T cell ones in AgB1 antigen by bioinformatics methods,which are significant in the further research of AgB1 antigenic and development of the immune diagnosis method.