| 用差异显示技术分离空心莲子草抗旱相关基因 |
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| 引用本文： | 高建明,罗才波,朱婷婷,何光源. 用差异显示技术分离空心莲子草抗旱相关基因[J]. 华中科技大学学报(自然科学版), 2007, 35(10): 116-118 |
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| 作者姓名： | 高建明 罗才波 朱婷婷 何光源 |
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| 作者单位： | 华中科技大学,生命科学与技术学院,湖北,武汉,430074;华中科技大学,生命科学与技术学院,湖北,武汉,430074;华中科技大学,生命科学与技术学院,湖北,武汉,430074;华中科技大学,生命科学与技术学院,湖北,武汉,430074 |
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| 基金项目： | 国家重点基础研究发展计划(973计划) |
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| 摘 要： | 采用mRNA差异显示技术,分离空心莲子草在干旱胁迫1 d后差异表达的基因,获得139个基因片段.经过Reverse Northern检测,初步证实有两个片段受干旱诱导表达上调.对其中一个片断克隆测序并进行核甘酸序列比对,显示与其他基因同源性都很低,表明可能是新的基因.半定量PCR技术证实该基因受干旱诱导表达上调.
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| 关 键 词： | 空心莲子草 差异显示 干旱 基因 |
| 文章编号： | 1671-4512（2007）10-0116-03 |
| 修稿时间： | 2006-07-17 |
Isolation of the drought responsive cDNA in Alternanthera philoxeroides by mRNA differential display |
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| Gao Jianming,Luo Caibo,Zhu Tingting,He Guangyuan. Isolation of the drought responsive cDNA in Alternanthera philoxeroides by mRNA differential display[J]. JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY.NATURE SCIENCE, 2007, 35(10): 116-118 |
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| Authors: | Gao Jianming Luo Caibo Zhu Tingting He Guangyuan |
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| Affiliation: | College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China |
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| Abstract: | The differential display technique was used to analyze genes differential expression in Alternanthera philoxeroides seedling under the condition of normal watering,drought for 24 h.One hundred and thirty-nine differentially expressed gene segments induced by drought were obtained and one of them was confirmed up-regulated by Reverse Northern and subsequently cloned,sequenced.Database showed that it has little similarity with other genes.Further confirmation of differential gene expression was made by semi-quantitative RT-PCR.This research provided basic information for the mechanism on drought resistance in Alternanthera philoxeroides. |
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| Keywords: | Alternanthera philoxeroides differential display drought gene |
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