A simplified method for isocratic HPLC analysis of polyamines |
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| Authors: | W L Whitmore T A Slotkin |
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| Institution: | (1) Department of Pharmacology, Duke University Medical Center, 27710 Durham, North Carolina, USA |
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| Abstract: | Summary A simple technique is described for the separation and analysis of polyamines in tissues and body fluids, utilizing precolumn clean-up on disposable CM-cellulose columns, followed by an automatable HPLC procedure. Complete separation and analysis takes 12–15 min per sample with sensitivity in the pmole range.Acknowledgments. Supported by USPHS HD-09713, EPA R811621010 and by Research Scientist Development Award DA-00006 to TAS. |
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| Keywords: | Polyamines putrescine spermidine spermine high pressure liquid chromatography |
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