前胡组织培养和细胞悬浮培养中胚状体发生及植株再生

前胡的种子幼苗切段在含有1mg/l2,4-D的(1/2)MS 固体培养基(大量元素减半)上,诱导产生愈伤组织。诱导两个半月的愈伤组织,在含有1mg/l6-BA+0.5mg/lIBA 或无激素的(1/2)MS 固体培养基上,分化出胚状体,再生成完整植株。愈伤组织在液体培养基中振荡分散及过滤制备成悬浮培养物,在含1mg/l2,4-D的液体培养基中,胚性细胞不断增殖。转移至含有0.05mg/l 2,4-D+0.05mg/l 6-BA 的(1/2)MS 液体培养基中,分化出胚状体,发育成完整植株。

EMBRYOGENESIS AND PLANT REGENERATION FROM TISSUE AND CELL CULTURE OF PEUCEDANUM DECURSIVUM(MIQ)MAXIM

Calli were produced from segments of seedling of Peucedanum dec- ursivum(Miq.)Maxim.cultured on the 1/2MS agar medium(with half quantity of macronutrients)containing 1 mg/1 2,4-D.After 2.5 mo- nths the calli were transferfed to 1/2MS agar medium containing 1 mg/l 6-BA+0.5 mg/l IBA or no phytohormones,they differentiated to em- bryoids and developed into plantlets.Cell suspension was established by shaking the calli in liquid.After filtering,the single cells and small cell groups were cultured in the 1/2MS liquid medium containing 1mg/l 2,4- D.Embryogenic cell clumps proliferated in this medium and different- iated into embryoids when transferred to a medium containing 0.05mg/1 2,4-D+0.05mg/l 6-BA.Embryoids developed into plantlets in 1 month aftef they were transferred to the solid medium with no phytohorm- ones.