Novel gene expressed during early embryogenesis of zebrafish identified by mRNA differential display |
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Authors: | Li Shuhong Guo Shaodong Sun Fangzhen |
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Institution: | (1) Institute of Developmental Biology, 100080 Beijing, China |
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Abstract: | Following the revelation of the molecular mechanism of morphogenesis in fruitfly, research on the molecular mechanism of morphogenesis
in vertebrate becomes the focus of developmental biology. The isolation of genes controlling the embryogenesis of zebrafish,
a vertebrate model animal, is considered as an initial step toward investigating this iasue. There are several approaches
that can be used to isolate developmental genes, each of which is suited to a particular situation. In this note, mRNA differential
display was utilized to demonstrate the mRNA differences among zebrafish embryos at 4, 5 and 6 h post fertilization (28.5°C,
corresponding to oblong, dome and shield stages, respectively, called blastula, gastrula and neumla in this note). One cDNA
tag that was specific to embryos at neurula stage was cloned and sequenced. After sequence comparison in Genbank, we found
that this cDNA tag represents a novel gene. The expression of this gene in the developing zebrafish embryos was examined by
whole mountin situ hybridization. The hybridization results confirmed that this gene was specifically expressed in zebrafish neurula embryos. |
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Keywords: | mRNA differential display zebrafish morphogenesis gene |
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