| TD1修饰绿色荧光蛋白透皮功能的研究 |
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| 引用本文： | 孙铮,温龙平. TD1修饰绿色荧光蛋白透皮功能的研究[J]. 中国科学技术大学学报, 2009, 39(4) |
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| 作者姓名： | 孙铮 温龙平 |
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| 作者单位： | 中国科学技术大学生命科学学院,合肥微尺度物质科学国家实验室,安徽合肥,230027 |
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| 基金项目： | 国家重点基础研究发展规划(973计划)，The cultivation fund of key scientific and technical innovation project |
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| 摘 要： | TD1作为一种含有11个氨基酸的短肽,具有良好的促进蛋白类大分子透皮的功能.过去的研究显示TD1可以有效协助胰岛素通过皮肤进入循环并最终降低血糖.在本研究中我们构建了一种TD1 N端修饰的GFP融合蛋白(TGFP). 我们的实验表明, 与TD1与GFP蛋白的混合物相比, TGFP具有更加良好的透皮功能.这一发现为透皮给药研究提供了一条新的途径,并对解释TD1透皮功能具有指导意义.
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| 关 键 词： | 透皮 绿色荧光蛋白(GFP) 融合蛋白(TGFP) |
Transdermal delivery of fusion green fluorescent protein mediated by covalently associated TD1 peptide |
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| SUN Zheng,WEN Long-ping. Transdermal delivery of fusion green fluorescent protein mediated by covalently associated TD1 peptide[J]. Journal of University of Science and Technology of China, 2009, 39(4) |
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| Authors: | SUN Zheng WEN Long-ping |
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| Abstract: | TD1 peptide, with the amino acid sequence of ACSSSPSKHCG, has been reported to have the potential ability to facilitate transdermal delivery of macromolecules such as insulin through skin. Here we report that TD1-GGS-eGFP (TGFP), or the N-terminal TD1 modified green fluorescent protein (GFP), compared to the unmodified GFP, is more capable of transporting through skin barrier in comparison with the unmodified GFP. This finding may provide a new way for transdermal drug delivery. It can also offer a new experimental methodology for the elucidation of the mechanism through which TD1 peptide facilitates transdermal delivery. |
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| Keywords: | TD1 TD1 transdermal green fluorescent protein (GFP) TD1-GGS-eGFP (TGFP) |
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