| Evolutionary Mode of Metallothioneins Inferred from Cd-MT Genes of Tetrahymena |
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| 作者姓名： | WAN Ming-liang FENG Wei-song MIAO Wei WONG Ming-hong |
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| 作者单位： | [1]State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academyof Sciences, Wuhan 430072, Hubei, China [2]Institute for Natural Resources and Environmental Management, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China |
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| 基金项目： | Supported by KSCX of Chinese Academy of Sciences Grant （SW-102） and the Allocation from the Earmaarked Grant for Hong Kong. |
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| 摘 要： | From Tetrahymena thermophila （strain BF5）, the coding region of Cd-MT gene was cloned and sequenced. and identified as MTT1 isoform. A serial duplicate structure is discovered in its amino acid sequence, which separates the coding region into three parts （Part 1：7-61; Part 2：64-118; Part 3：122-162）. The alignments among them and comparison with the corresponding parts of MT1 isoform suggest that MT1 and MTT1 isoforms both come from the same ancient gene that is homologous to Part 1, and Cd-MTs of Tetrahymena are aroused by such ancient gene duplication. The prediction of secondary structures and the analysis of the disulfide-bonding state of cysteine show that there are a lot of differences between MT1 and MTT1 isoforms, which maybe relate to their function mechanism.
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| 关 键 词： | 四膜虫 进化模式 Cd-MT基因 遗传特性 蛋白质 |
| 文章编号： | 1007-1202（2005）05-0925-06 |
| 收稿时间： | 2004-10-15 |
Evolutionary Mode of Metallothioneins Inferred from Cd-MT Genes of Tetrahymena |
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| WAN Ming-liang FENG Wei-song MIAO Wei WONG Ming-hong.Evolutionary Mode of Metallothioneins Inferred from Cd-MT Genes of Tetrahymena[J].Wuhan University Journal of Natural Sciences,2005,10(5):925-930. |
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| Authors: | WAN;Ming-liang;FENG;Wei-song;MIAO;Wei;WONG;Ming-hong |
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| Abstract: | From Tetrahymena thermophila (strain BF5), the coding region of Cd-MT gene was cloned and sequenced, and identified as MTT1 isoform. A serial duplicate structure is discovered in its amino acid sequence, which separates the coding region into three parts (Part 1: 7-61; Part 2: 64-118; Part 3: 122-162). The alignments among them and comparison with the corresponding parts of MT1 isoform suggest that MT1 and MTT1 isoforms both come from the same ancient gene that is homologous to Part 1, and Cd-MTs of Tetrahymena are aroused by such ancient gene duplication. The prediction of secondary structures and the analysis of the disulfide-bonding state of cysteine show that there are a lot of differences between MT1 and MTT1 isoforms, which maybe relate to their function mechanism. |
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| Keywords: | Tetrahymena tkermopkila metallothioneins evolutionary mode gene duplication protein spatial structure metal-binding sites |
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