竹叶总黄酮测定方法优化

为了建立一种快速、精确的竹叶总黄酮测定方法,采用以碱性样品溶液为参比溶液,以异荭草苷为标准品以及缩短加入显色试剂后的静置时间等措施,来对一般的比色法进行优化.该比色法在减少杂质干扰和排除芦丁标准品引起的测定误差的同时,使测定时间缩短了三分之一.且该方法具有良好的线性和精密度,平均加样回收率为100.14%,RSD为0.79%(n=5).结果表明,优化的比色法可用于竹叶总黄酮的测定.

Optimization of the determination method of the total flavonoids in bamboo leaves

In order to establish a rapid and accurate determination method for measurement of total flavonoids in bamboo leaves, a general colorimetric method was optimized by using the alkaline sample solution as the reference solution, using isoquertoside as the standard, and shortening the staining time after adding the staining reagent. The optimized colorimetric method reduced the measurement time by 1/3 while reducing the impurities interference and eliminating the measurement error caused by the rutin standard. Furthermore, the method provided good linearity and precision. The average recovery rate was 100.14%, and the RSD was 0.79%(n=5). The results showed that the optimized colorimetric method can be used for the determination of total flavonoids in bamboo leaves.