内蒙古地区2型糖尿病患者危险因素与脂联素基因多态性交互作用研究

目的：探讨内蒙古地区2型糖尿病患者危险因素与脂联素基因rs266729多态性交互作用。 方法：病例组来源于内蒙古医科大学附院以及内蒙古中蒙医院糖尿病患者共111例，对照组来源于内蒙古医科大学附属医院体检中心共106例。运用PCR-RFLP技术检测脂联素（ADIPOQ） 基因rs266729位点多态性。采用二元logistic回归方法分析脂联素基因rs266729多态性与T2DM的相关性，并且与环境因素进行交互作用分析。结果： ADIPOQ 基因rs266729多态性等位基因频率分布符合 Hardy-Weinberg 定律; T2DM基因型频率分布依次为 CC: 85.61% CG : 5.42%GG : 9.02%。校正 BMI之后，CG基因型人群是 CC基因型人群患T2DM的0.260倍，且有显著性意义( p=0.009)，而 GG基因型人群是 CC基因型人群患 T2DM的1.146倍， 无显著性意义( P=0.790)。 交互作用分析结果表明： rs266729位点与空腹血糖存在正交互作用；与收缩压、空腹 C肽、空腹胰岛素、生活环境存在负交互作用。结论：脂联素基因 rs266729多态性可以增加个体2型糖尿病罹患风险，且rs266729基因多态性与与空腹血糖、吸烟、酗酒、日常运动、日常情绪、生活紧张度、腰臀比、超重、糖尿病家族史这几项存在正交互作用，与收缩压、空腹 C肽、空腹胰岛素、生活环境存在负交互作用。

Study on the Interaction of Risk Factors and Adiponectin Gene Polymorphism in Type 2 Diabetes Mellitus in Inner Mongolia

Objective: To explore the interaction between risk factors of adiponectin gene rs266729 polymorphism and type 2 diabetes mellitus in Inner Mongolia. METHODS: A total of 111 cases of diabetic patients from the Affiliated Hospital of Inner Mongolia Medical University and Inner Mongolia Hospital of Chinese Medicine were included in the case group. The control group was from 106 cases of the physical examination center of the Affiliated Hospital of Inner Mongolia Medical University. The rs266729 polymorphism of adiponectin (ADIPOQ) gene was detected by PCR-RFLP. A binary logistic regression analysis was used to analyze the association between the adiponectin gene rs266729 polymorphism and T2DM, and interact with environmental factors. Results: The frequency distribution of the rs266729 polymorphism alleles of the ADIPOQ gene was in accordance with Hardy-Weinberg''s law; the frequency distribution of the T2DM genotype was CC: 85.61% CG: 5.42% GG: 9.02%. After adjusting for BMI, the CG genotype population was 0.260 times higher than that of T2DM in the CC genotype population (p=0.009), whereas the GG genotype population was 1.136 times higher than the CC genotype population in T2DM, with no significant significance. (P=0.790). The results of interaction analysis showed that there was a positive interaction between rs266729 locus and fasting blood glucose, and a negative interaction with systolic blood pressure, fasting C-peptide, fasting insulin, and living environment. Conclusion: The adiponectin gene rs266729 polymorphism can increase the risk of type 2 diabetes in individuals with rs266729 polymorphism and fasting blood glucose, smoking, alcoholism, daily exercise, daily mood, life tension, waist-to-hip ratio, overweight, The family history of diabetes has positive interactions with negative interactions with systolic blood pressure, fasting C-peptide, fasting insulin, and living conditions.