黄河三角洲湿地木霉分离与耐盐活性鉴定

木霉菌可以提高植物的抗逆性。为了获得耐盐活性好的木霉菌株,本研究从盐渍化严重的山东东营黄河三角洲湿地中采集土样和水样,分离并筛选了耐盐的木霉菌株,鉴定木霉菌株的耐盐活性。结果表明:黄河三角洲湿地48个样品共分离获得64株木霉,木霉数量与土壤深度呈负相关,与盐度没有明显相关性;通过不同浓度Na Cl胁迫下木霉菌株在固体培养基(PDA)和液体培养基(PDW)中的生长和产孢情况观察,从64株木霉中鉴定出6株具有高耐盐活性的木霉菌株;其中3株木霉(TW-304、TW-327和TW-353)在1 000 mmol/L Na Cl胁迫下,菌丝生长与对照相比,耐盐活性达到50%以上,具有极高的耐盐性;通过形态学和分子学鉴定显示TW-304、TW-327和TW-353三株木霉分别为哈茨木霉、深绿木霉、哈茨木霉。该研究筛选获得的耐盐木霉菌株为耐盐微生物菌剂的开发利用提供新的种质资源。

Isolation and Salt Tolerance Study of Trichoderma from the Yellow River Delta Wetland

Trichoderma can improve the plant stress resistance. In order to obtain the Trichoderma strains with high salt-tolerance activity, soil samples and water samples were collected from the salinized Yellow River Delta wetland in Dongying, Shandong Province, and the salt-tolerant Trichoderma was isolated and identified. The results show that 64 strains of Trichoderma were isolated from 48 samples, and the number of Trichoderma was negatively correlated with soil depth but not significant correlated with the salinity. Six strains of Trichoderma with high salt tolerance activity were identified through the observation of growth and sporulation in solid medium (PDA) and liquid medium (PDW) under different concentrations of NaCl stresses. Among them, the mycelial growth of 3 strains of Trichoderma spp. (TW-304, TW-327 and TW-353) under the stress of 1000 mM NaCl, reached more than 50% compared with the control, had extremely high salt tolerance. The 3 strains (TW-304, TW-327 and TW-353) were identified as Trichoderma harzianum, Trichoderma viridis and Trichoderma harzianum by morphological and molecular identification. The salt-tolerant Trichoderma strains obtained in this study provided new germplasm resources for the development and utilization of salt-tolerant microbial agents.