Microinjection of synthetic protein kinase inhibitor into single barnacle muscle fibers before and after cyclic AMP |
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Authors: | E E Bittar J Nwoga |
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Institution: | (1) Department of Physiology, University of Wisconsin, 53706 Madison, Wisconsin, USA;(2) Present address: Department of Physiology, Faculty of Medicine, University of Nigeria, Enugu, Nigeria |
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Abstract: | Summary Single muscle fibers from the barnacleBalanus nubilus have been used as a preparation to see if a synthetic 20-residue PKI (5-24)-peptide is able to block or reverse the stimulatory response of the ouabain-insensitive Na efflux to injected cAMP. The results obtained show that this peptide behaves as a powerful inhibitor of the cAMP-mediated response and is also able to partially reverse the sustained stimulation of the Na efflux observed in ouabain-poisoned fibers following the injection of subunit A of cholera toxin.Warmest thanks to Prof. E. H. Fischer and Kurt Diltz for a sample of sPK1. This work received support from NSF, the Graduate School Research Committee and from NIH through a General Research Support Grant to the University of Wisconsin Medical School. |
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Keywords: | Synthetic PKI cAMP sodium efflux barnacle muscle fibers |
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