离心—冷冻法制备梯度孔结构的真皮支架

胶原基真皮支架的结构和性能受多方面因素影响,比如除胶原外的其他主要材料、支架的孔径和孔隙率以及交联度等.采用离心—冷冻法结合冷冻干燥法制备梯度孔结构的真皮支架,表征支架的孔径和孔隙率,并以细胞培养检验其生物相容性,其中采用扫描电镜对上中下3部分支架结构及孔径进行表征,并通过细胞培养生长实验检测该支架材料的细胞粘附性能,以达到表征离心力的影响.结果显示,离心力对支架的微观结构产生了影响,支架的致密程度从下到上有明显的不同,具体表现在孔径上:上层孔径52±27.8μm,中层孔径57±8.7μm,下层孔径49±40.4μm.同时,细胞粘附实验表明,致密的下层更适合细胞的粘附生长.

Dermis Scaffold of Gradient Pore Structure Fabricated by Centrifugal-freeze Method

There are many factors controlling the microstructure and properties of collagen-based dermis scaffold,such as other main materials,the pore diameter and porosity as well as the cross-linking density.Dermis scaffold of gradient pore structure was fabricated by combination of centrifugal-freeze method and freeze-drying method.The pore diameter and porosity of the scaffold were investigated by scanning electron microscope.Biocompatibility and cell adhesion properties of the scaffold were tested by experiment of cell culture growth to characterize the influence of centrifugal force.The results show that the centrifugal force has important influence on the microstructure of dermis scaffold with different degree of densification from the bottom up on the three layers and the pore diameters are different from each other,like the upper is 52±27.8 μm,the middle is 57±8.7 μm and the lower is 49±40.4 μm.Moreover,the results of cell adhesion experiment show that the lower layer is more suitable for the adhesion and growth of cell among the three different structures.