Cloning and sequencing of Gtx-2 homeobox sequence

Murine genomic DNA was surveyed by polymerase chain reaction (PCR) to detect homeobox sequence of mammal. The PCR product (413 bp) was cloned and sequenced. The nucleotide sequence and the deduced amino acid sequence of a homeobox, which was isolated in this study, designated Gtx-2 were obtained. The result of sequence analysis showed that there is an intron between the nucleotides at position 138 and 139 in the Gtx-2 homeobox. Southern blot analysis revealed that Gtx-2 is likely to exist as a single copy in the murine genome. Comparison of the encoded polypeptide sequence with other homeodomains reveals that Gtx-2 has 96% and 58% identity to that of Gtx-1 and Tcl-3, respectively. Ma Xiaojun: born in 1941, Associate professor.