动物源性食品的氯霉素ELISA检测及分析

采用酶联免疫法检测了730例天津口岸进出口肉制品、水产品、乳制品和蜂蜜等几种动物源性食品中的氯霉素含量,对阳性结果经液相色谱-质谱联用EEC Cy3.6 confirming method进行确证,分析了其残留现状,并对英国RANDOX公司氯霉素检测试剂盒从检测范围、B/B050%抑制浓度、板内变异、板间变异、样品添加试验、实际样品检测、稳定性等方面各项指标进行试验.结果表明,试剂盒的检测低限为0.1 ng/mL,线性范围为0.05～4.7 ng/mL,B/B050%抑制浓度为0.4 ng/mL,样品加标回收率为80.5%～110%,板内变异系数小于5%,板间变异系数小于10%,试剂盒稳定性好.

Analysis for the ELISA Detection of Chloramphenicol Residues in Animal Food

Studied on screening,determination and confirmation of chloramphenicol(CAP) in four kinds of matricesi,ncluding meat productfi,shery products,milk productsh,oney etcb,y enzyme-linked immunoassay(ELISA) li,quid chromatography-tandem mass spectrometry(LC-MS2) have been carried out.The following parameters of kits were determined: detection capability,specificity,accuracya,pplicability and stability.Data obtained show that the limit of detection(LOD) is 0.1ng/mL.The linear plots are obtained between 0.05 and 4.7 ng/mL.The inhibited concentration(B/Bo50%) is 0.4ng/mL.The overall recoveries are between 80.5% and 110%;and the intra-and inter-precision values are within 5% and 10%.The stability of kit is good.