| 酶促半合成人胰岛素的副反应及纯化 |
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| 引用本文： | 刘钧松,汤钧,刘莹,李彬,周慧,李惟,卜凤泉. 酶促半合成人胰岛素的副反应及纯化[J]. 吉林大学学报(理学版), 1998, 0(1) |
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| 作者姓名： | 刘钧松 汤钧 刘莹 李彬 周慧 李惟 卜凤泉 |
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| 作者单位： | 吉林大学分子生物学系!长春，130023，吉林大学分子生物学系!长春，130023，吉林大学分子生物学系!长春，130023，吉林大学分子生物学系!长春，130023，吉林大学分子生物学系!长春，130023，吉林大学分子生物学系!长春，130023，白求恩医科大学应用基础医学研究所!长春，130021 |
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| 摘 要： | 研究酶促半合成人胰岛素的副反应．采用DEAE－SephadexA－25阴离子交换层析纯化合成人胰岛素中间体衍生物，去除脱八肽胰岛素以及人胰岛素类似物，精制酶促半合成人胰岛素．
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| 关 键 词： | 人胰岛素 脱八肽胰岛素 反相高效液相色谱 副反应 纯化 |
Side Reaction and Purification of the Enzyme-catalyzing Semi-synthesis of Human-insulin |
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| Liu Junsong, Tang Jun, Liu Ying, Li Bin, Zhou Hui, Li Wei. Side Reaction and Purification of the Enzyme-catalyzing Semi-synthesis of Human-insulin[J]. Journal of Jilin University: Sci Ed, 1998, 0(1) |
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| Authors: | Liu Junsong Tang Jun Liu Ying Li Bin Zhou Hui Li Wei |
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| Abstract: | Human-insulin is the most extensively used drug for curing,diabetes. Up to now, there have been two ways to produce human-insulin: making use of recombinant DNA technology and semisynthesis method, which is catalysed by proteases in specific organic media. The present paper deals with the some problems existed in the semi-synthesis of human-insulin catalysed by trypsin. The mainby-product was identified in the semi-synthesis of human-insulin. The small peak appeared in front of the main peak of human-insulin in RP-HPLC, which showed the same reserve time with the standardDOI. The present paper reports also a simple method for purifying human-insulin prepared by means of semi-synthesis method. The crude semi-synthesis human-insulin was purified by means of DEAESephadex A-25 ion-exchange chroma tography. The results showed that the purified product displayed a single band at the PAGE pattern, the same reserve time as the standard human-insulin in the RPHPLC. |
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| Keywords: | human-insulin DOI RP-HPLC side reaction purification |
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