基于聚氨基吡啶膜固定纳米金/硫堇/纳米金的过氧化氢生物传感器

研究了在铂丝电极上电聚合一层带正电的2氨基吡啶膜,然后再利用层层自组装技术固定纳米金、电子媒介体硫堇及辣根过氧化氢酶,从而制备了由辣根过氧化氢酶/纳米金/硫堇/纳米金/聚2氨基吡啶膜修饰的酶生物传感器.实验中探讨了聚合层数、温度、pH值等对电极响应的影响.结果表明该传感器在H2O2浓度6·0×10-7~1·3×10-3mol/L范围内呈线性响应,检出限为2·1×10-7mol/L.此外,该传感器具有好的稳定性和选择性,能有效排除抗坏血酸、柠檬酸、葡萄糖等常见物质的干扰.

An Amperometric Hydrogen Peroxide Biosensor Based on Poly 2-Aminopyridine/ Nano-Au and Thionine Modified Pt Electrode

A novel hydrogen peroxide biosensor has been prepared. HRP/nano-Au/thionine/nano-Au were constructed by the self-assembly technique on the electrode modified by poly 2-aminopyridine, which was electropolymerized onto a platinum(Pt) electrode to form a positively charged surface. The negatively charged nano-Au absorbed on poly 2-aminopyridine was employed as matrix to immobilize thionine as an electron mediator and horseradish peroxidase. The H2O2 biosensor prepared displayed a quick and sensitive response to hydrogen peroxide. The linear response of detection of the biosensor to hydrogen peroxide is in the concentration range of 6. 0 × 10-7 - 1.3 × 10-a mol/L with a detection limit of 2. 1 × 10-7 mol/L. Moreover, the studied biosensor exhibited good repeatability and high selectivity and it can effectively remove the interference of such common matter as the ascorbic acid citric acid glucose, etc.