不同品种黄皮果汁的品质及抗氧化能力

测定了20种黄皮果汁的可溶性固形物、可溶性糖、可滴定酸的质量分数及p H等基础品质指标以及抗坏血酸、总黄酮和总多酚等生物活性成分的质量浓度,并采用DPPH法和FRAP法评价其抗氧化能力,同时探讨了抗氧化能力与抗坏血酸、总黄酮、总多酚质量浓度的相关性.结果表明:不同黄皮品种间的可溶性固形物、可溶性糖、可滴定酸的质量分数和抗坏血酸、总黄酮、总多酚的质量浓度差异较大,其糖酸比、p H、DPPH自由基清除能力以及FRAP抗氧化能力差异也较大,基于上述理化指标的Q型聚类分析可将20个黄皮品种分成3大类群; DPPH自由基清除能力、FRAP抗氧化能力均与抗坏血酸、总黄酮和总多酚的质量浓度呈极显著的正相关(P<0.01),表明黄皮果汁的抗氧化作用与其所含的抗坏血酸、总黄酮和总多酚关系密切.

The Quality and Antioxidant Capacity of Fruit Juice of Different Wampee Cultivars

In this study, the basic quality parameters (pH, soluble solid content, soluble sugar content and titratable acid content) and the contents of major bioactive components (ascorbic acid, total flavonoids, and total phenolics) of fruit juice from 20 different wampee (Clausena lansium) cultivars were determined. Meanwhile their antioxidant activities were also evaluated based on 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and ferric reducing antioxidant power (FRAP) assays. Moreover, the correlation of antioxidant activities with ascorbic acid, total flavonoids and total phenolics contents were explored. The results showed that significant differences in pH, soluble solid content, soluble sugar content, titratable acid content, sugar/acid ratio, ascorbic acid content, total flavonoids content, total phenolics content, DPPH radical scavenging capacity and FRAP antioxidant capacity existed among the 20 wampee cultivars. The Q cluster analysis showed that the 20 wampee cultivars were divided into 3 groups based on the above physicochemical indices. Both DPPH scavenging capacity and FRAP antioxidant capacity were significantly positively correlated with the contents of ascorbic acid, total flavonoids and total phenolics, suggesting that antioxidation of wampee fruit juice was closely correlated with its bioactive composition of ascorbic acid, total flavonoids and total phenolics.