Quantitative structure activity relationship and toxicity mechanisms of chlorophenols on cells <Emphasis Type="Italic">in vitro</Emphasis> |
| |
Authors: | Jie?Jiang Jiangning?Chen Hongxia?Yu Feng?Zhang Email author" target="_blank">Junfeng?ZhangEmail author Liansheng?Wang |
| |
Institution: | (1) The State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, 210093 Nanjing, China;(2) The State Key Laboratory of Pollution Control and Resources Reuse, School of Environmental Science, Nanjing University, 210093 Nanjing, China |
| |
Abstract: | dimethylthiazd-2-yl)-2,5-diphenylentra- zolium bromide (MTT) reduction assay was used to investi-gate the acute toxicity of 8 different chlorophenols (CPs) on rat connective tissue fibroblast L929 cells and human liver cancer HepG2 cells. Combined with the data from Quantita-tive Structure Activity Relationship (QSAR) approach of CPs by using the octanol-water partition coefficients (Kow), an effective model was deduced to evaluate the cytotoxicity of these chemicals. Furthermore, the relationship between the structures of CPs and their cytotoxicity was proposed. The results show that 2-chlorophenol (2-CP), 4-chlorophenol (4-CP), 2,6-dichlorophenol (2,6-DCP), 2,4-dichlorophenol (2,4-DCP), 2,4,6-trichlorophenol (2,4,6-TCP) and 2,3,4-tri- chlorophenol (2,3,4-TCP) induced apoptosis, whereas, 2,3,5,6- tetrachlorophenol (2,3,5,6-TeCP) and pentachlorophenl (PCP) demonstrated more characteristic of necrosis than apoptosis. These results establish a good experimental base both for developing the comparative evaluation of toxicity of CPs in vitro and for elucidating the toxicity mechanisms of them. |
| |
Keywords: | chlorophenols (CPs) MTT assay QSAR cytotoxicity |
本文献已被 CNKI 维普 万方数据 SpringerLink 等数据库收录! |
| 点击此处可从《中国科学通报(英文版)》浏览原始摘要信息 |
| 点击此处可从《中国科学通报(英文版)》下载免费的PDF全文 |
|