水稻黄绿叶基因YGLOSH的定位克隆

本文利用~(60)Co γ射线诱变光温敏感型核不育系广占63S(GZ63S),在后代中获得一个稳定遗传的黄绿化叶色突变体黄广占63S,突变体从苗期至成熟期均显示叶片黄化特征.遗传分析表明该性状受一对隐性核基因控制,命名为yglosh.利用BSA方法分析突变体黄广占63S与正常绿叶对照蜀恢881构建的F2群体,将该黄化基因定位于水稻2号染色体分子标记RM279和Pm6之间,物理距离约68kb.定位区间的cDNA测序分析发现,突变体中LOC_Os02g05890基因发生单碱基突变,形成终止子提前终止该基因的翻译.转基因互补实验确定LOC_Os02g05890为目标基因,可能参与叶绿体发育或者叶绿素生物合成途径.

Fine Mapping and Clone a Yellow Green Leaf Gene (YGLOSH) in Rice

A leaf color mutant Huang guangzhan 63S was obtained by 60Co Gamma ray treating seeds of photoperiod and temperature sensitive genetic male sterility line guangzhan 63S,this mutation showed yellow-green leaves during the whole life,and the genetic analysis of F2 populations confirmed that this mutational character was controlled by a single recessive karyogene named yglosh.Analysis the F2 population builded by Huang guangzhan 63S and Shuhui 881 with BSA method,the gene was mapped between two microsatellite makers RM279 and Pm6 with physical distances about 68 kb.Through sequencing analysis of cDNA in this interval we found the LOS_Os02g05890 which had been confirmed as target gene by transgenie complementary experiment has single base mutation and generate a terminator to terminate the gene translation.The gene may participate in chloroplast development or chlorophyll biosynthetic pathway.