| 外源基因的导入对Synechococcus sp.PCC7942 SOD活性和同工酶谱的影响 |
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| 引用本文： | 徐虹,章军,谭维娜,林鉴,刘仁海,周克夫.外源基因的导入对Synechococcus sp.PCC7942 SOD活性和同工酶谱的影响[J].厦门大学学报(自然科学版),2004,43(3):398-401. |
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| 作者姓名： | 徐虹 章军 谭维娜 林鉴 刘仁海 周克夫 |
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| 作者单位： | 厦门大学生命科学学院,细胞生物学与肿瘤细胞工程教育部重点实验室,福建,厦门,361005 |
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| 基金项目： | 福建省自然科学基金(B0210001)，厦门市科技计划资助 |
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| 摘 要： | 外源DNA导入Synechococcus sp．PCC7942后．通过NBT光化还原和PAGE电泳检测发现转基因藻Synechococcus sp．PCC7942的SOD活性和同工酶谱发生了改变．若外源基因整合在受体细胞染色体DNA上，根据整合位点的不同则能提高或减弱Synechococcus sp．PCC7942的SOD活性，同时增加同工酶谱带．若外源基因不是整合在染色体上而是以质粒的形式存在，则只改变Synechococcus sp．PCC7942 SOD活性而不影响其同工酶谱．
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| 关 键 词： | 外源基因 基因导入 Synechococcus sp．PCC7942 SOD 超氧化物歧化酶 同工酶 转基因 |
| 文章编号： | 0438-0479(2004)03-0398-04 |
| 修稿时间： | 2003年9月15日 |
Effects of Introduction of Foreign Gene on Activity and Isoenzyme of Superoxide Dismutase of Synechococcus sp.PCC7942 |
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| XU Hong,ZHANG Jun,TAN Wei-na,LIN Jian,LIU Ren-hai,ZHOU Ke-fuumor Cell Engineering,School of Life Sciences,Xiamen University,Xiamen ,China.Effects of Introduction of Foreign Gene on Activity and Isoenzyme of Superoxide Dismutase of Synechococcus sp.PCC7942[J].Journal of Xiamen University(Natural Science),2004,43(3):398-401. |
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| Authors: | XU Hong ZHANG Jun TAN Wei-na LIN Jian LIU Ren-hai ZHOU Ke-fuumor Cell Engineering School of Life Sciences Xiamen University Xiamen China |
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| Institution: | XU Hong,ZHANG Jun,TAN Wei-na,LIN Jian,LIU Ren-hai,ZHOU Ke-fuumor Cell Engineering,School of Life Sciences,Xiamen University,Xiamen 361005,China) |
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| Abstract: | The activity and the isoenzyme of superoxide dismutase (SOD) in three types of transgenic Synechococcus sp. PCC7942 were analysed by photoreduction of NBT and PAGE electrophresis. The results showed that introduction of exogenous DNA does change the activities and numbers of SOD isoenzyme bands. If esogenous gene were integrated into chromosomal DNA of host cell, it can enhance or reduce enzyme activity and add iosenzyme bands of SOD in transformed Synechococcus sp .PCC7942. But if exogenous genes existed in shuttle plasmid and not integrated into chromosome of host cell, it can enhance activity of SOD, but does not change the number of SOD isoenzyme band. |
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| Keywords: | Synechococcus sp PCC7942 SOD isoenzyme transgene |
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