Construction and expression of retroviruses encoding dual drug resistance genes in human umbilical cord blood CD34+ cells |
| |
Authors: | Jun Yang Ying Wu Ping Ma Yanhua Zhao Ying You Susan E. Kane Ning Mao Yangpei Zhang |
| |
Affiliation: | (1) Institute of Blood Transfusion Medicine, 100850 Beijing, China;(2) Institute of Basic Medical Sciences, 100850 Beijing, China;(3) Department of Cetl and Tumor Biology, City of Hope National Medical Center, 91010, CA, USA |
| |
Abstract: | A series of retro viral vectors encoding humanmdr1 gene alone as wetl as in combination with either humanmgmt gene or human mutantSer 31-dhfr gene are engineered. The resultant retroviruses are used to transduce human umbilical cord blood CD34+ cetls. It has been shown that expression of dual drug resistance genes in transduced cetls confers a broad range of resistance to both kinds of corresponding drugs. These data suggest a rationale for the use of such double chemoresistance gene constructs in anin vivo model in which transduced hematopoietic cetls will acquire multiple protection against the cytotoxic side effects of combination chemotherapy and may have future application in chemoprotection of normal tissues, thus killing tumor cetls more effectivety. |
| |
Keywords: | tumor chemotherapy retrovirus drug resistance gene cord blood CD34+ cells |
本文献已被 SpringerLink 等数据库收录! |
| 点击此处可从《科学通报(英文版)》浏览原始摘要信息 |
|
点击此处可从《科学通报(英文版)》下载全文 |