| 分泌抗弓形虫单克隆抗体杂交瘤细胞株的建立 |
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| 引用本文： | 范文平,贺争鸣,卫礼,巩薇,吴惠英.分泌抗弓形虫单克隆抗体杂交瘤细胞株的建立[J].实验动物科学,1999,16(3):17-19. |
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| 作者姓名： | 范文平 贺争鸣 卫礼 巩薇 吴惠英 |
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| 作者单位： | 中国药品生物制品检定所,北京,100050 |
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| 摘 要： | 用弓形虫可溶性抗原免疫ＢＡＬＢ／ｃ 小鼠，取脾细胞，与Ｓｐ２／０ 细胞融合，获得３ 株能稳定分泌抗弓形虫单克隆抗体的细胞。细胞培养上清液和腹水中的单克隆抗体效价分别为１∶１６ ～２５６ 和１０ － ６ ～１０ － ７ 。单抗类型均为ＩｇＭ。杂交瘤细胞经液氮冻存１２ 个月后，复苏生长良好，分泌活性稳定。在ＭｃＡｂ－ Ｄｏｔ－ ＥＬＩＳＡ 实验中，所能测出可溶性抗原的最小浓度为１０ ｎｇ／ ｍＬ
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| 关 键 词： | 弓形虫 单克隆抗体 |
| 收稿时间： | 1999-09-25 |
| 修稿时间： | 1999年6月5日 |
Establishment of Hybridoma Cell Lines Secreting McAb to Toxoplasmosis |
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| Fan Wenping,He Zhengming,Wei Li,Gong Wei,Wu Huiying.Establishment of Hybridoma Cell Lines Secreting McAb to Toxoplasmosis[J].Shiyan Dongwu Kexue,1999,16(3):17-19. |
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| Authors: | Fan Wenping He Zhengming Wei Li Gong Wei Wu Huiying |
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| Institution: | National Institute for the Control of Pharmaceutical and Biological Products |
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| Abstract: | ? Three hybirdoma cell lines secreting monoclonal antibodies were raised by fusing of Sp2/0 cells with spleen cells of BALA/c mice immunized with the antigens of Toxoplasmosis. The titer of McAbs in culture supernatant and AF were 1∶16～256 and 10 -6 ～10 -7 seperately by ELISA,all of McAb were IgM class.The cell lines were preserved in liquid nitrogen for 12 months without any loss in immune reactivity. |
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| Keywords: | Toxoplasmosis\ McAb |
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