| 玉米自交系4112幼胚愈伤组织诱导的激素优化研究 |
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| 引用本文： | 王洪振,王延续,程军,程焉平,王淑范. 玉米自交系4112幼胚愈伤组织诱导的激素优化研究[J]. 松辽学刊, 2007, 28(3): 65-67,81 |
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| 作者姓名： | 王洪振 王延续 程军 程焉平 王淑范 |
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| 作者单位： | [1]吉林师范大学生命科学学院,吉林四平136000 [2]四平市园林处,吉林四平136000 |
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| 摘 要： | 以玉米自交系4112的幼胚为外植体,以MS为基本培养基,研究了不同质量浓度的2,4-D对愈伤组织诱导的影响.结果表明,2,4-D浓度在1mg/L时愈伤组织诱导效果较好.在此基础上,进一步研究不同2,4-D和KT组合诱导玉米自交系幼胚愈伤组织的效果.实验结果表明,对玉米自交系4112幼胚愈伤组织诱导的较适宜激素组合为MS＋2,4-D（1mg/L）＋KT（0.5mg/L）.
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| 关 键 词： | 玉米自交系 幼胚 愈伤组织的诱导 优化 |
| 文章编号： | 1000-1840-（2007）03-0065-03 |
| 收稿时间： | 2007-05-24 |
| 修稿时间： | 2007-05-24 |
Optimization of Phytohormone Combination on Callus Induction in Maize Inbred line 4112 from Immature Embryos |
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| WANG Hong-zhen,WANG Yan-xu,CHEN Jun,CHEN Yan-ping,WANG Shu-fan. Optimization of Phytohormone Combination on Callus Induction in Maize Inbred line 4112 from Immature Embryos[J]. Songliao Journal (Natural Science Edition), 2007, 28(3): 65-67,81 |
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| Authors: | WANG Hong-zhen WANG Yan-xu CHEN Jun CHEN Yan-ping WANG Shu-fan |
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| Affiliation: | 1,College of Life Science,Jilin Normal University, Siping 136000,China;2.Department of Siping Garden,Siping 136000,China |
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| Abstract: | Influence of different mass concentration of 2,4 - D on callus induction in maize inbred line 4112 from immature embryos with MS being used as basal culture medium was studied. The result showed that the proper effect of callus induction had been obtained with concentration of 2,4- D being 1 mg/L. Based on this result,effect of combination of 2,4 - D and KT on callus induction in maize inbred line from immature embryos was further studied. The result indicated that the proper phytohormone combination on callus induction in maize inbred line 4112 was Ms being used as basal culture medium with concentration of 2,4 - D being 1 mg/L and concentration of KT being 0.5 mg/L. |
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| Keywords: | maize inbred line im mature embryos callus induction, optimization |
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