| 食管鳞癌患者血清可溶性HLA-G分子表达水平及其临床意义 |
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| 引用本文： | 赵勇,郑建勇,王为忠. 食管鳞癌患者血清可溶性HLA-G分子表达水平及其临床意义[J]. 科学技术与工程, 2009, 9(8) |
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| 作者姓名： | 赵勇 郑建勇 王为忠 |
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| 作者单位： | 第四军医大学西京医院胃肠外科,西安,710032;第四军医大学西京医院胃肠外科,西安,710032;第四军医大学西京医院胃肠外科,西安,710032 |
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| 摘 要： | 研究可溶性人类白细胞抗原G分子(sHLA-G)在食管鳞癌患者血清中的水平及其临床意义.应用酶联免疫吸附测定(ELISA)法检测60例食管鳞癌患者及28例对照者血清中sHLA-G(sHLA-G1 和 HLA-G5)水平,免疫组化S-P法检测对应的食管鳞瘤患者癌组织及对照组正常食管组织中HLA-G的表达,应用逆转录聚合酶链式反应(RT-PCR)法检测组织中HLA-GmRNA及HLA-G5mRNA的表达情况.结果食管鳞癌患者血清sHLA-G平均水平(15.04 u/mL)较对照(6.81 u/mL)升高(P=0.006);血清sHLA-G水平与肿瘤患者临床病理特征无相关性;HLA-G在60例癌组织表达率为70%且与肿瘤的分化程度(P=0.033)及淋巴结转移相关(P=0.035),正常食管组织无HLA-G表达;HLA-GmRNA和HLA-G5mRNA在20例HLA-G表达阳性的食管鳞癌组织中的均有表达,正常食管组织无HLA-GmRNA和HLA-G5mRNA表达.说明食管鳞癌组织可异常表达HLA-G分子,联合检测血清sHLA-G水平及HLA-G分子可作为临床食管癌的诊断和判断预后的指标.
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| 关 键 词： | 食管癌 鳞形细胞癌 可溶性人类白细胞抗原G |
Expression of Serum Soluble HLA-G in Esophageal Squamous Cell Carcinoma and its Clinical Significance |
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| ZHAO Yong,ZHENG Jian-yong,WANG Wei-zhong. Expression of Serum Soluble HLA-G in Esophageal Squamous Cell Carcinoma and its Clinical Significance[J]. Science Technology and Engineering, 2009, 9(8) |
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| Authors: | ZHAO Yong ZHENG Jian-yong WANG Wei-zhong |
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| Affiliation: | Department of Gastrointestinal Surgery;Xijing Hospital;Fourth military Medical university;Xi;an 710032;P.R.China |
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| Abstract: | To investigate the serum levels of sHLA-G in patients with esophageal squamous cell carcinoma and the relationship between sHLA-G and clinicopathological characteristics,the serum levels of sHLA-G(sHLA-G1 and HLA-G5) were measured by enzyme linked immunosorbent assay(ELISA) in 60 patients with esophageal squamous cell carcinoma(ESCC) and in 28 healthy subjects respectively.HLA-G protein expression was determined with S-P immunohistochemical staining,HLA-GmRNA and HLA-G5mRNA were detctedd with reverse transc... |
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| Keywords: | esophageal carcinoma squamous cell carcinoma sHLA-G |
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