利用SSR 标记正确判断小麦杂交种中的杂株

 正确判断杂交种中混有的杂株, 是准确鉴定小麦杂交种种子纯度的前提。在分析180 份杂交组合的基础上, 以“京麦1100”为例, 提出了利用SSR 标记正确判断小麦杂交种中杂株的方法:1)筛选双亲间有多态性的引物不少于5 对;2)利用该引物检测亲本及杂交种的基因型, 并按照杂交种基因型记录原则记录每个个体的基因型;3)正确判断真实杂交种基因型:不论亲本是否存在非纯合位点现象, 只要某个体同时具备父母本的基因型, 均视为真实杂交种的基因型;4)判断杂株:当某个体在多个SSR 位点上与真实杂交种基因型不同时, 判断为杂株。

Correct judgment method of contaminated plants in hybrid wheat using SSR markers

Correct judgment of contaminated plants in wheat hybrid is the premise for accurate identification of seed purity. In this study, taking the hybrid wheat variety "Jingmai1100" as an example, we propose a method for judging contaminated plants in hybrid wheat using SSR markers on the basis of analysis of 180 wheat hybrids: 1) Not less than five polymorphic SSR markers on parents of different genotypes should be screened; 2) Polymorphic SSR primers are used to detect the genotype of parents and hybrids, and each plan's genotype is recorded according to the recording principle of hybrid genotypes; 3) True hybrid genotypes should be judged accurately, with the following principle: No matter whether there is the homozygous locus in parents, as long as the individual has both parents' genotype, it should be regarded as a true hybrid genotype; 4) Judgment of contaminated plants in hybrids: When the individual is different from true hybrid on multiple SSR loci, it could be regarded as a contaminated plant.