证病结合的异常黑胆质型阿尔茨海默病(AD)模型的建立及行为学改变

 在前期已建立的异常黑胆质载体动物模型的基础上,采用聚集态Aβ1-40 双海马定向注射制备证病结合的异常黑胆质型阿尔茨海默病(AD)模型,并以Morris 水迷宫及跳台实验验证证病结合模型的行为学改变特点。结果表明,各组大鼠饮食、水量及体重变化:单纯(AD)组、异常黑胆质证组饮食、水量较正常对照组增加(P<0.05);证病结合的异常黑胆质型AD 模型组饮食、水量较异常黑胆质组减少(P<0.01);单纯AD 组、异常黑胆质证组、证病结合组体重较正常对照组均减轻(P<0.01)。各组大鼠Morris 水迷宫空间探索试验经过有效区域次数:与正常对照组比较,单纯AD 组、异常黑胆质证组、证病结合组经过有效区域次数均减少(P<0.01);与单纯AD 组相比,证病结合组经过有效区域次数明显减少(P<0.05)。各组大鼠跳台测试:与空白对照组比较,单纯AD 组、异常黑胆质证组和证病结合组反应期延长,潜伏期缩短,错误次数增多,学习记忆成绩明显降低(P<0.01);证病结合组与单纯痴呆组相比略低,但无统计学差异(P>0.05)。由此得出,在异常黑胆质载体大鼠模型的基础上,采用聚集态Aβ1-40 双海马定向注射制备证病结合的异常黑胆质型AD 模型,不仅动物体表特征的变化符合维医体液证候改变特点,通过行为学验证后学习记忆改变特点又符合西医疾病的特点。

Developing Alzheimer's disease rat model carrying abnormal savda syndrome and observing its behavioral changes

This paper developed an Alzheimer's disease rat model carrying abnormal Savda syndrome and studied its behavioral changes. First of all, an abnormal Savda syndrome rat model was developed by using the classical method then β-Amyloid 1-40 was injected into hippocampus to establish Alzheimer's disease in these rats. After observing the biological characteristics and testing behavioral changes with Morris water maze test and step down test, the abilities of learning and memorizing of different model groups were compared. Compared with the normal group, the daily food and water intake was increased in the abnormal Savda syndrome group and the AD group (P<0.01), the body weigh was decreased in the abnormal Savda syndrome group, the AD group and the AD carrying abnormal Savda syndrome group (P<0.01). The numbers of crossing the effective area were reduced in the abnormal Savda syndrome group, the AD group and the AD carrying abnormal Savda syndrome group (P<0.01) with respect to the normal group. The number of crossing the effective area was smaller in the AD carrying abnormal Savda syndrome group (P<0.05) than that in the AD group. The learning and memory scores were significantly lower in the abnormal savda syndrome group, the AD group and the AD carrying abnormal Savda syndrome group (P<0.01) than those in the normal group. Compared with the AD group, the learning and memory scores slightly lowered in the AD carrying abnormal Savda syndrome group without any significant statistical difference(P > 0.05). In this research, the Alzheimer's disease carrying abnormal Savda syndrome rat model was developed successfully.