春小麦重组自交系及多种SSR标记构建遗传图谱

 以春小麦重组自交系(RIL)宁春4 号×宁春27 号为作图群体,利用SSR 标记构建小麦遗传连锁图谱。结果表明,用1001对SSR 引物选出亲本间表现多态性的引物307 对,多态性频率为30.7%。利用307 对多态性引物对RIL 群体进行分析,共检测到266 个多态性标记位点。通过χ²检测(P<0.05),有147 个SSR 标记表现为偏分离,偏分离率为55.3%,129 个偏向母本宁春4号,其偏分离位点主要分布在B 和D 基因组上。用Mapmaker 3.0 和Mapdraw 2.1 软件将266 个SSR 位点绘制在小麦遗传连锁图上,该图谱覆盖小麦基因组全长2187.79 cM,标记间的平均遗传距离为8.22 cM。

Construction of wheat linkage map using a set of spring wheat recombinant inbred lines and different SSR markers

The recombinant inbred lines (RILs) between Ningchun 4 and Ningchun 27 are used as the donors for the construction of the spring wheat genetic linkage map by different SSR markers. It is revealed that 307 pairs of the SSR markers in total are available to show the SSR polymorphism differences between the two parents among 1001 pairs of SSR markers originally selected. The polymorphism frequency is 30.7%. The 307 pairs of the SSR primers with the polymorphisms between Ningchun 4 and Ningchun 27 are applied to further analyze the RIL population, and 266 loci with polymorphism are detected by the primers mentioned above. The χ² test (P<0.05) shows that 147 SSR markers see a significant segregation distortion of 55.3%, mostly distributed on the B and D genomes. Among them, the segregated distortions of 129 SSR markers are resulted from the female parent of Ningchun 4. By using the software of Mapmaker 3.0 and Mapdraw 2.1, 266 SSR markers are assigned on the linkage genetic map of the wheat, which covers about 2187.79 cM of the wheat genome with an average distance of 8.22 cM between markers.