维药异常黑胆质成熟剂对异常黑胆质型肝癌模型作用机制的血清代谢组学研究

 依托核磁共振氢谱(¹H-NMR)技术,探讨异常黑胆质成熟剂(ASM)对异常黑胆质型肝癌模型代谢谱的变化及其作用机制。根据维吾尔医学理论,建立维吾尔医异常黑胆质型肝癌病证大鼠模型,运用核磁共振氢谱技术检测以不同剂量ASM 灌胃大鼠的血清代谢物含量变化,分段积分后采用正交偏最小二乘判别(OPLS-DA)对¹H-NMR 谱数据进行模式识别分析。将1HNMR谱信息用PLS-DA 分析可以把异常黑胆质型肝癌组和ASM 低、中、高剂量组之间大鼠血清代谢谱变化区分开,发现与异常黑胆质肝癌组相比,在ASM 低剂量组大鼠的血清中α-葡萄糖,β-葡萄糖和牛磺酸的含量上升,而在ASM 中剂量组大鼠的血清中同样这三者也有升高,并且一些必需氨基酸,如亮氨酸、缬氨酸、丙氨酸、组氨酸含量升高,乳酸降低,并且差异有统计学意义(P<0.05),而在ASM 高剂量组大鼠的血清中变化无此趋势。异常黑胆质成熟剂通过提高支链氨基酸的含量,加速糖异生提高能量供应,降低乳酸堆积来调节异常黑胆质型肝癌模型的氨基酸代谢,糖代谢等能量代谢紊乱。

Effect of abnormal savda munziq on abnormal savda rat with hepatocarci based on serum metabonomic analysis

Using nuclear magnetic resonance spectroscopy, this paper studies the effect of abnormal savda munziq (ASM) on serum metabolic changes and the mechanism. According to the theory of traditional Uighur medicine, an abnormal savda rat model is established. ¹H-NMR technique is used to determinate serum metabolites in different doses of ASM. After break points, orthogonal partial least-squares discriminant analysis (OPLS-DA) is dopted for pattern recognition analysis of ¹H-NMR spectrum to distinguish changes in metabolic profiling of abnormal savda rat with hepatocarcinoma between the low, medium and high dose groups. Compared with abnormal savda rat with hepatocarcinoma, increased concentration levels of α-glucose, β-glucose, taurine are found in the ASM low dose group. For the serum of rats in the ASM medium dose group, this is also ture while lactic acid is decreased and some essential amino acids are increased. However, no such changes are seen in the serum of the ASM high dose group. Abnormal savda munziq may regulate amino acid metabolism, glucose metabolism such as energy metabolism of abnormal savda rat model with hepatocarcinoma by increasing the content of branched-chain amino acids, accelerating gluconeogenesis to increase energy supply, and reducing accumulation of lactic.