Effects of La3+ and Gd3+ on Ca2+ influx in rat hepatoma H-35 cells |
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Authors: | Xinmin Li Wangfun Fong Pengfei Kwok Mengsu Yang Patrick C. L. Wong Jiazuan Ni |
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Affiliation: | (1) Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 130022 Changchun, China;(2) Department of Biology and Chemistry, City University of Hong Kong, Hong Kong, China;(3) Department of Biochemistry, The University of Hong Kong, Hong Kong, China |
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Abstract: | Effects of La3+ and Gd3+ on Ca2+ influx were investigated in rat hepatoma H-35 cells by measuring the initial rate of45Ca2+ uptake. It was found that the maximum initial rate of Ca2+ uptake was increased six-to ten-fold at low concentrations of La3+ and Gd3+. Kinetic analyses by measuring the initial rate of Ca2+ influx at different external Ca2+ concentrations indicated the existence of two intracellular exchangeable components in the basal Ca2+ system, with low and high affinities for Ca2+, and only one class of Ca2+ binding sites was observed in the La3+-or Gd3+-treated cells. For high affinity, La3+ and Gd3+ increased both kinetic parametersK m andV max of basai Ca2+ influx. La3+ and Gd3+ compete directly with Ca2+ for Ca2+ binding site for low affinity. The kinetics is competitive. |
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Keywords: | La3+ Gd3+ rat hepatoma H-35 cells Ca2+ influx |
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