| 桂北五步蛇纤溶酶金属蛋白酶原基因的初步研究 |
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| 引用本文： | 杨海波,樊晓晖,何先保,黄企光,黎肇炎,Franc Gubensek.桂北五步蛇纤溶酶金属蛋白酶原基因的初步研究[J].广西科学,2000,7(4):307-308,318. |
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| 作者姓名： | 杨海波 樊晓晖 何先保 黄企光 黎肇炎 Franc Gubensek |
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| 作者单位： | 1. 广西医科大学微生物教研室南宁市滨湖路6号 530021
2. Dept of Biochemistry and Molecular Biology, Jozef Stefan Institute,Slovenia |
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| 基金项目： | 广西青年科学基金，桂青自 9912018， |
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| 摘 要： | 从桂北五步蛇毒腺中抽提蛇毒总ＲＮＡ，采用一步法（ＲＴ－ＰＣＲ和ＰＣＲ在同一试管内进行）扩增纤溶酶金属蛋白酶原基因，并进行电泳检测，可见３条特异的ＤＮＡ条带，分别约为１．５Ｋｂ、１．３Ｋｂ和８００ｂｐ，利用平端连接的方法将其中的８００ｂｐＰＣＲ产物克隆至ｐＧＥＭ－Ｔ Ｅａｓｙ载体，挑选白色菌落，用酶切和ＰＣＲ法对其进行鉴定。
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| 关 键 词： | 五步蛇 纤溶酶金属蛋白酶原 基因克隆 一步法 |
| 收稿时间： | 2000/5/18 0:00:00 |
| 修稿时间： | 2000/7/17 0:00:00 |
Primary Study of cDNA Encoding Fibrinolysin Metalloproteinase from the Venom of Agkistrodon acutus |
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| Yang Haibo,Fan Xiaohui,He Xianbao,Huang Qiguang,Li Zhaoyan and Franc Gubensek.Primary Study of cDNA Encoding Fibrinolysin Metalloproteinase from the Venom of Agkistrodon acutus[J].Guangxi Sciences,2000,7(4):307-308,318. |
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| Authors: | Yang Haibo Fan Xiaohui He Xianbao Huang Qiguang Li Zhaoyan and Franc Gubensek |
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| Institution: | Franc Gubensek ** Yang Haibo Fan Xiaohui He Xianbao Huang Qiguang Li Zhaoyan |
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| Abstract: | One step method was used to extract total RNA from the venom of Agkistrodon acutus which was found in the northern mountain area of Guangxi province. The cDNAs encoding fibrinolysin metalloproteinase were amplified by one step method (RT-PCR and PCR reactions occurred in the same test tube). The PCR product is about 1.5 Kb,1.3 Kb and 800bp.The 800 bp PCR product was cloned into the pGEM-T Easy vector. Plasmid DNA was obtained from positive clones, identified by means of digestion with EcoR I and PCR reaction. |
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| Keywords: | Agkistrodon acutus fibrinolysin metalloproteinase PCR gene clone |
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