影响黄瓜愈伤组织诱导的因素研究

以新津11号、绿宝和金宝218三个品种黄瓜的无菌苗子叶和下胚轴为外植体,通过组织培养技术,利用外源激素IAA和6-BA诱导愈伤组织,研究基因型、外植体和激素配比对愈伤组织诱导的影响。结果表明,不同基因型的黄瓜在愈伤组织形成的时间以及激素的最佳浓度选择上呈现出明显的差异,并且不同的外植体表现了不同的最佳激素水平以及愈伤组织形态,IAA最佳激素水平范围为0.2~0.3 mg/L,6-BA最佳激素水平范围为1.2~3 mg/L。

Impact factors on cucumber callus induction

We address the impacts of genotypes,explants and hormones on callus induction with cotyledons and hypocotyl from three different cucumbers,Xinjin 11,Lübao and Jinbao 218 as explants and exogenous hormones IAA and 6-BA inducing callus in MS media.Results show that callus formation time and optimal hormonal concentration selection exhibit significant distinctions for different cucumber genotypes.Moreover,different explants demonstrate different optimal hormone level and callus formation.Optimal hormonal concentration of IAA is 0.2 ～ 0.3 mg/L,and that of 6-BA is 1.2 ～ 3 mg/L.