| 一种基于水解过程的多糖快速鉴别方法 |
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| 引用本文： | 马耀宏,;孟庆军,;杨艳,;杨俊慧,;史建国,;张利群,;郑岚,;刘伟霞. 一种基于水解过程的多糖快速鉴别方法[J]. 山东科学, 2014, 27(4): 12-16. DOI: 10.3976/j.issn.1002-4026.2014.04.003 |
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| 作者姓名： | 马耀宏, 孟庆军, 杨艳, 杨俊慧, 史建国, 张利群, 郑岚, 刘伟霞 |
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| 作者单位： | 1山东省科学院生物研究所,山东省生物传感器重点实验室，山东 济南 250014;2山东农业大学, 山东 泰安 271018；3.山东省计算中心，山东 济南 250014 |
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| 基金项目： | 国家高技术研究发展计划（863计划）（2012AA021201）;山东省自主创新重大科技专项（20122501） |
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| 摘 要： | 应用生物传感器记录树舌、灵芝和虫草多糖水解过程的还原糖(还原性末端数)和葡萄糖含量的实时变化,研究水溶性多糖水解过程中还原糖(还原性末端数)和葡萄糖的水解过程变化规律。研究表明,同一种多糖水解图谱稳定,不同种多糖图谱特异性明显,通过水解过程图谱的比对分析,可以实现对树舌、灵芝和虫草多糖的快速鉴别。
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| 关 键 词： | 水解过程 葡萄糖 还原糖 多糖 |
| 收稿时间： | 2014-02-17 |
A hydrolysis process based rapid identification approach for polysaccharides |
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| MA Yao-Hong,MENG Qing-Jun,YANG Yan,YANG Jun-Hui,SHI Jian-Guo,ZHANG Li-Qun,ZHENG Lan,LIU Wei-Xia. A hydrolysis process based rapid identification approach for polysaccharides[J]. Shandong Science, 2014, 27(4): 12-16. DOI: 10.3976/j.issn.1002-4026.2014.04.003 |
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| Authors: | MA Yao-Hong MENG Qing-Jun YANG Yan YANG Jun-Hui SHI Jian-Guo ZHANG Li-Qun ZHENG Lan LIU Wei-Xia |
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| Affiliation: | 1. Shandong Provincial Key Laboratory of Biosensors, Biology Institute, Shandong Academy of Sciences,;Jinan 250014, China;2. Shandong Agricultural University, Taian 271018, China；3.Shandong Computer Science Center,Jinan 250014,China |
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| Abstract: | We employed biosensors to record real-time variations of reducing sugar and glucose contents in hydrolysis process of Ganoderma applanatum Pat,Ganoderma lucidum and Cordyceps polysaccharide.We further investigated the variation law of the hydrolysis process of reducing sugar and glucose in hydrolysis process of water-soluble polysaccharide.Results show that hydrolysis process map is stable for the same kind of polysaccharide,but the specificity of hydrolysis process map is significant for different kinds of polysaccharide.Rapid identification of Ganoderma applanatum Pat,Ganoderma lucidum,and Cordyceps polysaccharide can be implemented by the comparison analysis of hydrolysis process map. |
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| Keywords: | polysaccharide hydrolysis process glucose reducing sugar |
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