| 松枯梢病菌RAPD分子水平遗传距离分析 |
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| 引用本文： | 吴小芹,熊大斌.松枯梢病菌RAPD分子水平遗传距离分析[J].南京林业大学学报(自然科学版),2006,30(1):13-16. |
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| 作者姓名： | 吴小芹 熊大斌 |
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| 作者单位： | 1. 南京林业大学森林资源与环境学院,江苏,南京,210037
2. 江苏省林业局,江苏,南京,210036 |
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| 摘 要： | 对来自中国、美国、英国、南非和智利的松枯梢病菌23个菌株进行RAPD分析。用12个引物共扩增出135个RAPD标记,其中多态性标记占96.3%。各菌株间的Nei相似系数UPGMA法聚类结果表明,国内外23个菌株可大致分为3个类群:来自智利的CWS41与所有菌株的遗传关系最远;来自中国的F2和J2次之;其他的国外菌株与国内菌株的遗传关系则较近。此次实验未将来自美国的B型菌株与其他菌株区分开。
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| 关 键 词： | 松枯梢病菌 随机扩增多态性DNA 遗传相关 |
| 文章编号： | 1000-2006(2006)01-0013-04 |
| 收稿时间： | 2004-12-15 |
| 修稿时间： | 2005-05-30 |
RAPD Analysis on Genetic Relationships Among Sphaeropsis sapinea Isolates |
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| WU Xiao-qin,XIONG Da-bin.RAPD Analysis on Genetic Relationships Among Sphaeropsis sapinea Isolates[J].Journal of Nanjing Forestry University(Natural Sciences ),2006,30(1):13-16. |
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| Authors: | WU Xiao-qin XIONG Da-bin |
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| Institution: | 1. College of Forest Resources and Environment Nanjing Forestry University , Nanjing 210037, China;2. Jiangsu Forestry Administration, Nanjing 210036, China |
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| Abstract: | Genetic relationships were studied among 23 isolates of Sphaeropsis sapinea(collected) from China,United States,England, South Africa and Chile by using RAPD analysis method.135 scorable DNA fragments were amplified with twelve random primers by PCR(Polymerase Chain Reaction) technique and the polymorphic score was 96.3%.The genetic dendrogram based on RAPD analysis showed that the all S.sapinea isolates could be clustered into three groups.The isolate CWS41 from Chile was separated genetically as a first group away from other isolates,and isolates F2 and J2 from China were as the second group.The third RAPD group accommodated other rest isolates(including B morphotype CWS43 from United States). |
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| Keywords: | Sphaeropsis sapinea RAPD Genetic relationship |
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