| CoA产生菌的诱变育种及发酵条件研究 |
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| 引用本文： | 赵炯,刘祖同,夏丽群.CoA产生菌的诱变育种及发酵条件研究[J].清华大学学报(自然科学版),1991(6). |
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| 作者姓名： | 赵炯 刘祖同 夏丽群 |
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| 作者单位： | 清华大学生物科学与技术系
(赵炯，刘祖同)，华北制药厂(夏丽群) |
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| 摘 要： | 研究了ＣｏＡ产生菌诱变育种及摇瓶发酵条件。以产氨短杆菌（Ｂｒｅｖｉｂａｃ－ｔｅｒｉｕｍ ａｍｍｏｎｉａｇｅｎｅｓ）ＡＳ１．８４４为出发菌株， 经单菌落分离获得了 ＢＳ１．８４４菌株，再经过紫外和 ＮＴＧ诱变，获得了对 ＴＭＴＤ具有抗性的 ＣＴ４００变异株， ＣｏＡ产量为 ５３５ ｕ／ｍＬ，比原亲株提高了 ２６９％。实验了不同的培养基组分，获得了最适培养基。采用两步补料法能显著提高 ＣｏＡ产量，比原一步补料提高 ３３％。不加热提取工艺能有效降低色素含量， ＣｏＡ得率略有增加。实验选用了 ４种阳离子型表面活性剂，以 ＣＰＣ对积累 ＣＯＡ效果最好。
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| 关 键 词： | 产氨短杆菌 诱变育种 CoA发酵 |
Study on the Breeding of CoA- Producing Strain and Its Fermentation |
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| Zhao Jiong,Liu Zutong.Study on the Breeding of CoA- Producing Strain and Its Fermentation[J].Journal of Tsinghua University(Science and Technology),1991(6). |
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| Authors: | Zhao Jiong Liu Zutong |
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| Abstract: | A CoA-producing strain CT400 was derived from Brevibacterium ammoniagenes AS 1. 844 by combination treatment with ultraviolet light and NTG. CT400 has resistance to TMTD, and the yield of CoA by this mutant reaches 535 u/mL (1. 3 g/L), which is by 269% higher than that of its ancestor. The optimum medium was obtained through experiments. A maximum 33 % increase was obtained by the supplementation of substrate in two steps as compared to that in one step. No-heating separation reduced the colour substance, and also increased the yield of CoA. The stimulative effect of surfactants on the production of CoA is described. |
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| Keywords: | Brevibacterium ammoniagenes breeding CoA fermentation |
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