Analysis for the dorsalization potency of the animal blastomeres of the 16 cell stageXenopus embryo |
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Authors: | Qinghua Tao Xiaoyan Ding |
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Institution: | (1) Present address: Shanghai Institute of Cell Biology, Chinese Academy of Sciences, 200031 Shanghai, China |
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Abstract: | The dorso-ventral axis ofXenopus embryo is established after fertilization. Blastula stage blastomeres acquire different identities as they have inherited
different maternal materials which distribute radial symmetrically along the animal vegetal aixs in the full-grown oocyte,
and are rearranged by the cortical rotation triggered by fertilization. The vegetal blastmeres demonstrate the different dorsalization
potencies in the previous transplantation experiments. The data of blastomere explanting and RT-PCR analyzing indicate that
the dorsal ventral bias also exists among the animal blastomeres even during the early blastula stage. |
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Keywords: | Xenopus embryo dorso-ventral axis blastomere explantation gene expression RT-PCR |
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