| The induction of Sinorhizobium meliloti C4-dicarboxylate transport system （Dct） is regulated by oxygen concentration |
| |
| 引用本文： | WEN Jin NAN Beiyan Fergal O＇Gara WANG Yiping. The induction of Sinorhizobium meliloti C4-dicarboxylate transport system （Dct） is regulated by oxygen concentration[J]. 科学通报(英文版), 2005, 50(17): 1891-1895. DOI: 10.1369/982004-174 |
| |
| 作者姓名： | WEN Jin NAN Beiyan Fergal O＇Gara WANG Yiping |
| |
| 作者单位： | [1]National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China [2]Microbiology Department, University College Cork, National Unversity Ireland, Cork, Ireland |
| |
| 摘 要： | The Sinorhizobium meliloti C4-dicarboxylate transport （Dct） system is essential for symbiotic nitrogen fixation. The dctA gene, encoding the C4-dicarboxylate permease, is expressed in both free living and symbiotic cells. But in free living cells expression of dctD and dctB is absolutely required for the expression of dctA. In this study, in order to investigate the effect of oxygen concentration on the induction of Dct system, E. coli DH5a strain which carries the plasmid-encoded dctABD operon was used in tube assays. It was found that the specific induction of Dct system oc- curred only at a certain depth under the surface of M63-0.6% agar media, suggesting that Dct system could respond to oxygen concentration during succinate-induced expression. Furthermore, when measured at different oxygen concentrations, the highest expression level was observed at oxygen concentration of 2%. Thus, we predict that in addition to dicarboxylates, the induction of Dct system may also regulated by oxygen concentration.
|
| 关 键 词： | 黄香草木犀 根瘤菌 氧浓度 Dct系统 |
| 收稿时间： | 2004-08-13 |
| 修稿时间： | 2004-08-132005-08-08 |
The induction of Sinorhizobium meliloti C4-dicarboxylate transport system (Dct) is regulated by oxygen concentration |
| |
| Wen Jin,Nan Beiyan,Fergal O’Gara,Wang Yiping. The induction of Sinorhizobium meliloti C4-dicarboxylate transport system (Dct) is regulated by oxygen concentration[J]. Chinese science bulletin, 2005, 50(17): 1891-1895. DOI: 10.1369/982004-174 |
| |
| Authors: | Wen Jin Nan Beiyan Fergal O’Gara Wang Yiping |
| |
| Affiliation: | 1. National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, 100871, Beijing, China
2. Microbiology Department, University College Cork, National University Ireland, Cork, Ireland
|
| |
| Abstract: | The Sinorhizobium meliloti C4-dicarboxylate transport (Dct) system is essential for symbiotic nitrogen fixation. The dctA gene, encoding the C4-dicarboxylate permease, is expressed in both free living and symbiotic cells. But in free living cells expression of dctD and dctB is absolutely required for the expression of dctA. In this study, in order to investigate the effect of oxygen concentration on the induction of Dct system, K coli DH5α strain which carries the plasmid-encoded dctABD operon was used in tube assays. It was found that the specific induction of Dct system occurred only at a certain depth under the surface of M63-0.6% agar media, suggesting that Dct system could respond to oxygen concentration during succinate-induced expression. Furthermore, when measured at different oxygen concentrations, the highest expression level was observed at oxygen concentration of 2%. Thus, we predict that in addition to dicarboxylates, the induction of Dct system may also regulated by oxygen concentration. |
| |
| Keywords: | Sinorhizobium meliloti Dct system oxygen concentration. |
| 本文献已被 CNKI 维普 万方数据 SpringerLink 等数据库收录！ |
| 点击此处可从《科学通报(英文版)》浏览原始摘要信息 |
|
点击此处可从《科学通报(英文版)》下载全文 |
|
