| Reconstruction of HaSNPV with helicoverpa hormone receptor 3 |
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| 作者姓名： | 扈进冬 Shao Honglian Zhang Yubao Fu Qiang Sun Chen Wang Jinxing Zhao Xiaofan |
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| 作者单位： | [1]School of Life Sciences, Shandong University, Jinan 250100, P.R. China [2]School of Meclicine, Shangdong University, Jinan 250100, P.R. China |
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| 基金项目： | 国家自然科学基金
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国家高技术研究发展计划(863计划)
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山东省自然科学基金 |
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| 摘 要： | In order to develop a more efficient virus for controlling the cotton bollworm Helicoverpa armigera, Helicoverpa hormone receptor 3 （HHR3）, which is involved in the ecdysteroid regulatory pathway, was used to genetically modify wild HaSNPV. HaSNPV-HHR3 budded virus and occlusion body virus were constructed in three steps： preparation of pFastBacHaPhpP10-HHR3 donor plasmid, transposition of HHR3 into the HaBacHZ8 bacmid, and transfection of HzAM1 cells to get HaSNPV-HHR3 virus.HHR3 was proved to be expressed in the HaSNPV-HHR3 virus infected HzAM1 cells by immunoblotting. Results of bioassay indicated that the body weight of the HaSNPV-HHR3 infected larvae was lower than the larvae infected with wild virus and uninfected normal larvae, which suggests that HaSNPV-HHR3 delayed larval growth.
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| 关 键 词： | HaSNPV 激素受体 棉铃虫 鳞翅类 |
| 修稿时间： | 2006-06-13 |
Reconstruction of HaSNPV with helicoverpa hormone receptor 3 |
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| Hu Jindong,Shao Honglian,Zhang Yubao,Fu Qiang,Sun Chen,Wang Jinxing,Zhao Xiaofan.Reconstruction of HaSNPV with helicoverpa hormone receptor 3[J].High Technology Letters,2007,13(3):327-331. |
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| Authors: | Hu Jindong Shao Honglian Zhang Yubao Fu Qiang Sun Chen Wang Jinxing Zhao Xiaofan |
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| Institution: | 1. School of Life Sciences, Shandong University, Jinan 250100, P.R. China
2. School of Life Sciences, Shandong University, Jinan 250100, P.R. China;School of Meclicine, Shangdong University, Jinan 250100, P.R.China |
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| Abstract: | In order to develop a more efficient virus for controlling the cotton bollworm Helicoverpa armigera,Helicoverpa hormone receptor 3 (HHR3), which is involved in the ecdysteroid regulatory pathway, was used to genetically modify wild HaSNPV. HaSNPV-HHR3 budded virus and occlusion body virus were constructed in three steps: preparation of pFastBacHaPhpP10-HHR3 donor plasmid, transposition of HHR3 into the HaBacHZ8 bacmid, and transfection of HzAM1 cells to get HaSNPV-HHR3 virus. HHR3was proved to be expressed in the HaSNPV-HHR3 virus infected HzAM1 cells by immunoblotting. Results of bioassay indicated that the body weight of the HaSNPV-HHR3 infected larvae was lower than the larvae infected with wild virus and uninfected normal larvae, which suggests that HaSNPV-HHR3 delayed larval growth. |
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| Keywords: | lepidoptera noctuidae Helicoverpa armigera Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus (HaSNPV) Helicoverpa hormone receptor 3 (HHR3) genetical manipulation bioassay |
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