Purification and immobilization of human carbonic anhydrase B by using polyacrylamide gel

Summary Human erythrocyte carbonic anhydrase B was purified and immobilized in polyacrylamide gel. As compared to the soluble enzyme, the immobilized enzyme was considerably more resistant to heat and sulphanilamide action.Acknowledgments. I thank Prof. R. L. Brahmachary for providing me all the laboratory facilities to carry out the investigation.