| 流动注射化学发光法测定棉酚 |
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| 引用本文： | 赵梅,卫贝,郝芳. 流动注射化学发光法测定棉酚[J]. 山西师范大学学报：自然科学版, 2012, 0(2): 52-54 |
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| 作者姓名： | 赵梅 卫贝 郝芳 |
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| 作者单位： | 山西师范大学校医院;山西师范大学化学与材料科学学院 |
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| 基金项目： | 山西师范大学校科学研究基金资助(YS09007) |
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| 摘 要： | NaOH介质中,铁氰化钾可以氧化棉酚产生弱的化学发光,罗丹明6G有强的增敏作用.基于此,结合流动注射分析技术,建立了一种化学发光法测定棉酚的新方法.在最佳优化条件下,该方法测定棉酚的线性范围为2.0×10^-8mol/L～2.0×10^-6mol/L,检出限为1.4×10^-9mol/L（3σ）,相对标准偏差为2.0%.将该方法应用于棉籽油和血清中棉酚含量的测定,结果满意.
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| 关 键 词： | 流动注射 化学发光 铁氰化钾 罗丹明6G 棉酚 |
Determination of Gossypol by Flow Injection Chemiluminescence |
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| ZHAO Mei,WEI Bei,HAO Fang. Determination of Gossypol by Flow Injection Chemiluminescence[J]. Journal of Shanxi Teachers University, 2012, 0(2): 52-54 |
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| Authors: | ZHAO Mei WEI Bei HAO Fang |
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| Affiliation: | 1.School hospital,Shanxi Normal University,Linfen 041004,Shanxi,China; 2.School of Chemistry and Material Science,Shanxi Normal University,Linfen 041004,Shanxi,China) |
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| Abstract: | A simple,sensitive and inexpensive flow injection chemiluminescence method for the determination of gossypol had been discovered.It was based on the fact that the weak chemiluminescent reaction of potassium ferricyanide with gossypol in alkaline medium,which was enhanced by rhodamine 6G.A lot of optimized experimental conditions had been studied in detail.The CL intensity was linearly related to the concentration of gossypol in the range of 2.0×10-8 mol/L~2.0×10-6 mol/L and the detection limit was 1.4×10-9 mol/L(3σ).The relative standard deviation(RSD) for the technique was 2.0 %(n = 11,c = 4.0×10-7 mol/L).The proposed method had been triumphantly applied to the determination of gossypol in cottonseed oil and human serum with satisfactory results. |
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| Keywords: | flow-injection chemiluminescence potassium ferricyanide Rhodamine 6G gossypol |
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