| 中国美利奴羊PRLR基因外显子10SSCP多态性与部分繁殖性状的关联分析 |
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| 引用本文： | 吴洪宾,吴荷群,王遵宝,余鹏,赵宗胜,张文祥. 中国美利奴羊PRLR基因外显子10SSCP多态性与部分繁殖性状的关联分析[J]. 石河子大学学报(自然科学版), 2011, 29(1) |
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| 作者姓名： | 吴洪宾 吴荷群 王遵宝 余鹏 赵宗胜 张文祥 |
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| 作者单位： | 石河子大学动物科技学院;新疆兵团农六师五家渠市畜牧兽医工作站;石河子大学生命科学学院; |
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| 摘 要： | 以催乳素受体基因(PRLR)作为绵羊繁殖性状的候选基因,运用PCR-SSCP方法对PRLR外显子10的多态性进行检测,并对其多态性与绵羊部分繁殖性状进行了关联分析。结果表明:在PRLR外显子10的2个基因片段存在PCR-SSCP多态,PRLR1扩增片段存在3种基因型,PRLR2扩增片段存在4种基因型;Hardy-Weinberg平衡检验显示,中国美利奴羊群体在2个位点都处于平衡状态;CE和DD基因型中国美利奴羊产羔数均值比CD和DE型多0.31只(P<0.05);AA基因型中国美利奴羊初生重均值比BB型重0.66 kg(P<0.05);DE基因型中国美利奴羊3月龄重均值比CD型重2.42 kg(P<0.05)。
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| 关 键 词： | 绵羊 PRLR 繁殖性状 PCR-SSCP |
Relationship between Polymorphism of Exon 10 of Prolactin Receptor Gene and Reproduction Trait of Chinese Merion Sheep |
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| WU Hongbin,WU Hequn,WANG Zunbao,YU Peng,ZHAO Zongsheng,ZHANG Wenxiang. Relationship between Polymorphism of Exon 10 of Prolactin Receptor Gene and Reproduction Trait of Chinese Merion Sheep[J]. Journal of Shihezi University(Natural Science), 2011, 29(1) |
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| Authors: | WU Hongbin WU Hequn WANG Zunbao YU Peng ZHAO Zongsheng ZHANG Wenxiang |
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| Affiliation: | WU Hongbin1,WU Hequn2,WANG Zunbao1,YU Peng3,ZHAO Zongsheng1,ZHANG Wenxiang1(1 College of Animal Science and Technology,Shihezi University,Shihezi 832003,China,2 Animal Husbandry and Veterinary Station at Agricultural Construction Division 6 in Xinjiang,Wujiaqu 831300,3 College of Life Science,Shihezi Univesity,China) |
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| Abstract: | This study selected prolactin receptor(PRLR) as the candidate gene of sheep reprodution trait,detected the polymorphism of exon 10 of prolactin receptor gene and analyzed the relationship between polymorphism of exon 10 of PRLR between reproduction trait of sheep.The result showed that there were two transitions in exon 10 of sheep PRLR gene,which were three genetypes of PRLR1 and four genetypes of PRLP2;both of the two sites accorded with Hardy Weinberg equilibrium in Chinese Merino sheep.The correlation a... |
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| Keywords: | sheep PRLR reproduction trait PCR-SSCP |
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