两亲卟啉在Triton X-100胶束中的紫外可见光谱研究

利用紫外可见光谱研究了两亲的对一四羟基苯基卟啉(THPP)在非离子表面活性剂聚氧乙烯基醚(Triton X-100)胶束中的光谱行为，随着体系pH的增加，THPP的Soret带从424nm红移至439nm，四个Q带(520nm，561nm，595nm，653nm)消失而在580nm和667nnm处产生两个与金属卟啉的吸收光谱极为相似的新峰，这一现象说明卟啉发生了去质子化，在pH7．54时，其光谱类似于在THF溶剂中，而在pH12．46时，其光谱又类似于强碱水溶液，由此可知，可以通过改变体系pH来控制卟啉环在胶束中的增溶位置从而实现卟啉的跨膜转移。

UV-Visible Spectral Behavior of Amphiphilic Porphyrin in Triton X-100 Micelle

By taking advantage of the deprotonation property of amphiphilic porphyrin-meso-tetrakis p-hydroxyphenyl porphyrin(THPP) and the spectral sensitivity to the microenvironment of the porphyrin moiety,a pH-controlled transmembrane process was investigated by means of UV-visible spectra.With increasing of pH values,the soret band shifted from 424 nm to 439 nm and the four Q bands (520 nm,(561 nm),595 nm,653 nm) disappeared while two new Q bands appeared at 580 nm and 667 nm,which was similar to metal-porphyrin.The phenomenon suggests that the deprotonation of porphyrin took place.In addition,the spectral characteristic to THPP in neutral and in basic Triton X-100 micelle was similar to that in THF and basic aqueous,indicating that the solubilizing location of THPP changed from inner to the outer of the Triton X-100 micelle.The result reveals that changing pH can control transmembrane process of THPP.