Study on the interaction between Jak3 and IL-2R γ using the yeast two-hybrid system |
| |
摘 要: | Both interleukin-2 (IL-2) receptor γ subunit and non-receptor tyrosine kinase Jak3 play important roles in IL-2 physiological functions. Jak3 has been known to bind IL-2Rγ and the interaction is very important for IL-2 signaling. In order to find the domains directly involved in the interaction between IL-2Rγ and Jak3, various deletion mutants have been constructed and their interaction has been studied using the yeast two-hybrid system. Results show that the JH3-JH7 region of Jak3 N-terminal can bind to intracellular domain of IL-2Ry directly and the intact structure of JH3-JH7 region is necessary for this combination. In addition, the region from 305 to 317 amino acid residues near the C-terminal of IL-2Ry plays a critical role in this interaction .
|
关 键 词: | IL-2Rγ Jak3 yeast two-hybrid system interaction. |
收稿时间: | 1999-02-10 |
本文献已被 SpringerLink 等数据库收录! |
| 点击此处可从《科学通报(英文版)》浏览原始摘要信息 |
|
点击此处可从《科学通报(英文版)》下载全文 |